There are number of plants and classical Polyherbal formulations reported in market but standardization to support their efficacy are lacking. Large numbers of therapies are based on the herbs and their formulations. At present Validation of such plants has become too much essential to represent them in the modern context with their efficacy. In the present investigation, one Polyherbal formulation (Neorise granules) consists of aqueous extract of Brahmi (Centella asiatica) and Shankhapushpi (Evolvulus alsinoides). Here estimation is done by isolating Asiaticoside, which is quantified by HPTLC method. All the above mentioned plants were identified by its morphological and microscopical analysis and physicochemical evaluation. Preliminary phytochemical screening revealed the presence of alkaloid, tannin, phenolic compounds, Saponin, Flavanoids, Glycosides, Triterpenoid and bitter principles. TLC densitometric methods were developed using HPTLC for the quantification of Asiaticoside present in polyherbal formulation. Asiaticoside content was 0.0054 ± 0.01% in Polyherbal formulation (Neorise granules). The method was validated in terms of Linearity, Precision, and Limit of detection, Limit of quantification, Accuracy and specificity.
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